A METHODOLOGICAL COMPARISON AMONG DNA SOURCE TYPES FOR MOOSE GENOTYPING

Tessa L. Unger, Ron A. Moen, Jared L. Strasburg

Abstract


Population genetic analyses for moose have been based on DNA extracted from blood and other body tissues. Non-invasive sampling of fecal pellets is another potential source of DNA. We compared DNA extraction from blood, liver tissue, and fecal pellet samples from moose in Minnesota and Yellowstone National Park, USA. Extracted DNA from all source types was sufficient for genotyping using 15 microsatellites. DNA extracted from fecal pellets was of lower quality and quantity than DNA extracted from blood and tissue. We provide comparisons of efficiency and effectiveness of DNA extraction protocols for blood, tissue, and fecal pellets, and demonstrate the suitability of using DNA extracted from non-invasively sampled material in moose.


Keywords


DNA extraction; fecal pellets; genotyping; microsatellites; moose; polymerase chain reaction; population genetics

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